bio rad cfx connect real time pcr detection system (Bio-Rad)
Bio-Rad is a verified supplier 
Bio-Rad manufactures this product 
98
Structured Review
Bio-Rad
bio rad cfx connect real time pcr detection system
Bio Rad Cfx Connect Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 4019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+cfx/CFX+Maestro+Software/pmc12930076-116-7-7
Average 98 stars, based on 4019 article reviews
Bio Rad Cfx Connect Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 4019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+rad+cfx/CFX+Maestro+Software/pmc12930076-116-7-7
Average 98 stars, based on 4019 article reviews
bio rad cfx connect real time pcr detection system - by Bioz Stars,
2026-10
98/100 stars
Images
1) Product Images from "Screening for novel chemical scaffolds targeting PCNA identifies the Hsp90alpha inhibitor SNX-2112"
Article Title: Screening for novel chemical scaffolds targeting PCNA identifies the Hsp90alpha inhibitor SNX-2112
Journal: Current Research in Structural Biology
doi: 10.1016/j.crstbi.2026.100183
Figure Legend Snippet: PCNA AI-CADD molecule thermal shift assay screening hits . AI-CADD screening: Left , plot of normalized melt curves of compounds + PCNA melt. Center , normalized derivative RFU of melt curve data. The DMSO control is plotted in black dashed lines. Right , . Calculated Δ Tm values of 19 AI-CADD hits. Thermal shift assays were performed using a Bio-Rad CFX Connect Real-Time PCR Detection System. and the final concentration of recombinant purified PCNA was 9 μM, and the final concentration of each compound was 1 mM. The reaction plates were heated from 25 °C to 95 °C with heating increments of 0.5 °C/min. Fluorescence intensity was measured within the excitation/emission ranges 470–505/540–700 nm. A negative control of 100% DMSO was included in each assay to obtain an apo- PCNA melting temperature (Tm apo ). The melting temperature of each reaction well was extrapolated from the midpoint of excitation of the produced melt curves by the CFX Maestro Software (Bio-Rad). The thermal shift values were calculated using the following equation: ΔTm = Tm + compound – Tm apo .
Techniques Used: Thermal Shift Assay, Control, Real-time Polymerase Chain Reaction, Concentration Assay, Recombinant, Purification, Fluorescence, Negative Control, Produced, Maestro Software
Related Articles
Real-time Polymerase Chain Reaction:Article Title: Antibiotic resistance genes detected in lichens: insights from Cladonia stellaris. Article Snippet: .. We conducted qPCR analyses using a Article Title: Investigation into the sensitivity of adipocytes mediated by the major vault protein (MVP) to chemotherapy for triple-negative breast cancer Article Snippet: To quantify messenger RNA (mRNA), complementary DNA was prepared using the PrimeScript TM RT Reagent Kit (Takara). .. Real-time PCR was performed on a Article Title: Identification and functional characterization of an AMD associated c-ABL binding SNP streak within the ARMS2 gene promoter region Article Snippet: Complementary DNA (cDNA) was synthesized from total RNA using either the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) or the iScriptTM cDNA Synthesis Kit (Bio-Rad). .. Quantitative PCR (qPCR) was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) or SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a SYBR Green Assay:Article Title: Investigation into the sensitivity of adipocytes mediated by the major vault protein (MVP) to chemotherapy for triple-negative breast cancer Article Snippet: To quantify messenger RNA (mRNA), complementary DNA was prepared using the PrimeScript TM RT Reagent Kit (Takara). .. Real-time PCR was performed on a Article Title: Albumin-binding dendrimer-conjugated siRNA enables safe and effective gene silencing throughout the central nervous system Article Snippet: RNA was extracted using RNA Clean & Concentrator Kit (Zymo Research, Cat. #R1016) and quantified on a Nanodrop (ThermoFisher Scientific). .. For reverse transcription, 1 μg of RNA was used with the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Cat. #4368813) per the manufacturer’s protocol. qRT-PCR was carried out in technical duplicates using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. #1725122) on Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity. Article Snippet: Data processing and mass image reconstruction were conducted with IMAGEREVEALTM MS (Shimadzu). qRT-PCR Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1708891). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1725120) on a Article Title: Identification and functional characterization of an AMD associated c-ABL binding SNP streak within the ARMS2 gene promoter region Article Snippet: Complementary DNA (cDNA) was synthesized from total RNA using either the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) or the iScriptTM cDNA Synthesis Kit (Bio-Rad). .. Quantitative PCR (qPCR) was performed using iTaq Universal SYBR Green Supermix (Bio-Rad) or SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity Article Snippet: Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1725120). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1708891) on a Quantitative RT-PCR:Article Title: Design of bifunctional RNA-binding compounds that can modulate siRNA hydrophobicity for deep penetration and gene silencing in the retina Article Snippet: One microliter of siRNA fraction solution (from supernation or pellet) was aliquoted for stemloop reverse transcription (MR101, Vazyme), followed by real-time qPCR quantification using the AceQ qPCR Probe Master Mix (Vazyme, Q112). .. RT-qPCR was performed on a Article Title: Albumin-binding dendrimer-conjugated siRNA enables safe and effective gene silencing throughout the central nervous system Article Snippet: RNA was extracted using RNA Clean & Concentrator Kit (Zymo Research, Cat. #R1016) and quantified on a Nanodrop (ThermoFisher Scientific). .. For reverse transcription, 1 μg of RNA was used with the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Cat. #4368813) per the manufacturer’s protocol. qRT-PCR was carried out in technical duplicates using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. #1725122) on Maestro Software:Article Title: Design of bifunctional RNA-binding compounds that can modulate siRNA hydrophobicity for deep penetration and gene silencing in the retina Article Snippet: One microliter of siRNA fraction solution (from supernation or pellet) was aliquoted for stemloop reverse transcription (MR101, Vazyme), followed by real-time qPCR quantification using the AceQ qPCR Probe Master Mix (Vazyme, Q112). .. RT-qPCR was performed on a Reverse Transcription:Article Title: Albumin-binding dendrimer-conjugated siRNA enables safe and effective gene silencing throughout the central nervous system Article Snippet: RNA was extracted using RNA Clean & Concentrator Kit (Zymo Research, Cat. #R1016) and quantified on a Nanodrop (ThermoFisher Scientific). .. For reverse transcription, 1 μg of RNA was used with the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Cat. #4368813) per the manufacturer’s protocol. qRT-PCR was carried out in technical duplicates using iTaq Universal SYBR Green Supermix (Bio-Rad, Cat. #1725122) on Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity. Article Snippet: Data processing and mass image reconstruction were conducted with IMAGEREVEALTM MS (Shimadzu). qRT-PCR Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1708891). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1725120) on a Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity Article Snippet: Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1725120). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1708891) on a Polymerase Chain Reaction:Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity. Article Snippet: Data processing and mass image reconstruction were conducted with IMAGEREVEALTM MS (Shimadzu). qRT-PCR Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1708891). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1725120) on a Article Title: Microbiome of the Proximal Small Intestine in Patients with Acute Pancreatitis. Article Snippet: A second round of amplification was performed using standard Illumina indexing primers with adapters. .. Both rounds of PCR were performed using PCR buffer (Eurogen, Moscow, Russia) and a Article Title: Very long chain sphingolipids govern brain myelination by regulating oligodendrocyte differentiation and membrane microdomain integrity Article Snippet: Total RNAs were extracted from brain using total RNA extraction kit (TIANGEN, Cat No: DP419) and cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Cat No 1725120). .. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the SYBR Green PCR kit (Bio-Rad, Cat No 1708891) on a |